The closure will allow crews to remove surfacing, place pipe and bring the roadway up to grade with a temporary surface until paving operations can get underway.
Interstate 80 between Cheyenne and Laramie is now open to all traffic in both directions, but Interstate 25 between Cheyenne and Wheatland remains closed.
The Wyoming Department of Transportation says the interstates in southeast Wyoming will likely remain closed overnight after being closed due to winter conditions.